Peptides supplied by Pure Peptides UK come in a lyophilized (freeze-dried) powder form. This state ensures maximum stability and shelf life. However, to use them in liquid assays, they must be properly reconstituted.

## What You Need

- **Bacteriostatic Water:** Sterile water containing 0.9% benzyl alcohol. The alcohol prevents bacterial growth once the vial is punctured.
- **Insulin Syringes:** For precise measurement and gentle handling of the peptide.
- **Alcohol Prep Pads:** To sterilize the rubber stoppers.

## The Reconstitution Process

1. **Sterilize:** Wipe the rubber stoppers of both the peptide vial and the Bacteriostatic water vial with alcohol.
2. **Draw Solvent:** Use an insulin syringe to draw the desired amount of Bacteriostatic water.
3. **Inject Slowly:** Insert the needle into the peptide vial. **Crucial step:** Inject the water slowly, aiming for the glass wall of the vial, not directly onto the delicate powder.
4. **Mix Gently:** Do not shake! Gently swirl the vial until the powder is completely dissolved and the solution is clear.

## Storage Rules

- **Unreconstituted:** Can be stored at room temperature for short periods, but long-term storage should be in a freezer (-20°C).
- **Reconstituted:** Must be stored in a refrigerator (2°C to 8°C) and protected from light. Properly reconstituted peptides typically remain stable for 3 to 4 weeks.

Stock up on Bacteriostatic Water, PBS, and Insulin Syringes at Pure Peptides UK.

*Disclaimer: For in vitro laboratory research use only.*